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Fig. 1 | BMC Cardiovascular Disorders

Fig. 1

From: Procyanidin B2 alleviates oxidized low-density lipoprotein-induced cell injury, inflammation, monocyte chemotaxis, and oxidative stress by inhibiting the nuclear factor kappa-B pathway in human umbilical vein endothelial cells

Fig. 1

Ox-LDL induced cell injury and the expression of LOX-1 and adhesion molecules in HUVECs. (A) HUVECs were treated with DMSO or ox-LDL (100 µg/ml) for 24–48 h. Untreated cells functioned as the control group. Cell viability was examined by CCK-8 assay. (B-D) HUVECs were treated with DMSO or ox-LDL (100 µg/ml) for 24 h. DMSO-treated cells functioned as the control group. (B) Cell apoptotic pattern was tested by flow cytometry. (C) The mRNA levels of LOX-1, MCP-1, VCAM-1, and ICAM-1 were detected by RT-qPCR assay. (D) The protein level of LOX-1 was measured through western blot assay. (E) The secretion levels of MCP-1, VCAM-1 and ICAM-1 were gauged by ELISA. n = 3 independent biological replicates in A-D. *P < 0.05, **P < 0.01, ***P < 0.001

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BMC Cardiovascular Disorders

ISSN: 1471-2261

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