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Fig. 5 | BMC Cardiovascular Disorders

Fig. 5

From: Procyanidin B2 alleviates oxidized low-density lipoprotein-induced cell injury, inflammation, monocyte chemotaxis, and oxidative stress by inhibiting the nuclear factor kappa-B pathway in human umbilical vein endothelial cells

Fig. 5

PCB2 inhibited the expression of CXCL1 and CXCL8 and recruitment of THP-1 cells in ox-LDL-treated HUVECs. (A and B) HUVECs were stimulated with ox-LDL (100 µg/ml) or DMSO vehicle for 24 h and treated with or without PCB2 (1 µg/ml) for an additional 12 h. (A) CXCL1 and CXCL8 mRNA levels were measured by RT-qPCR assay. (B) CXCL1 and CXCL8 secretion levels were tested by ELISA assay. (C) HUVECs were treated with ox-LDL (100 µg/ml) for 24 h alone or along with PCB2 (1 µg/ml) for another 12 h. Then, cell supernatants were collected and added to the low chambers. THP-1 cells were added to the upper chambers. Five hours later, THP-1 cells in the low chambers were stained, imaged, and counted. n = 3 independent biological replicates in A-C. *P < 0.05, **P < 0.01

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BMC Cardiovascular Disorders

ISSN: 1471-2261

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