Fig. 5

IL-6R as a novel target gene of miR-155 A) Bioinformatics analysis suggested that IL-6R is a new target gene of miR-155. B) 20 nM of negative control or miR-155 mimic and 150 ng of pmirGLO plasmid containing WT or 3′ -UTR mutation of target gene were co-transfected into HEK293T cells, and dual luciferase activity was detected 48 h later. ***P < 0.001, miR-155 mutant vs. miR-155 WT. C) Negative control or miR-155 mimic was transfected into Cardiomyocytes at 100 nM, and the mRNA expression of IL-6R in cardiomyocytes was detected by qRT-PCR after 48 h. **P < 0.01, miR-155 vs. NC treatment. D) Negative control or miR-155 mimic was transfected into cardiomyocytes at 100 nM, and the IL-6R in cardiomyocytes was analyzed by immunoblotting after 48 h. Each experiment in this figure was replicated more than three times