Fig. 3

Construction of a THP-1 lipid-loaded model and screening of the LY86 knockdown sequence were performed. THP-1 cells were treated with 50 µg/ml ox-LDL for 24 h, and the cropped blot and quantification of LY86 protein. (Figures A and C). The expression level of LY86 was also evaluated by Western blotting and qRT-PCR after transfection of THP-1 cells with si-LY86-01 sequence for 24 h (Figures B, D, E). The results are presented as mean ± SD (n = 3); ^*P<0.05, ^**P<0.01, ^***P<0.001