Fig. 4

LY86 effectively attenuates ox-LDL-induced lipid accumulation in THP-1 macrophages. (A) Representative images of oil red O staining in THP-1 cells (×40 magnification). (B, C) The cropped blot and quantification of intracellular HMGCR, SREBP2, GRP78, and LY86 after 24-hour knockdown treatment with si-LY86 followed by 24-hour stimulation with ox-LDL; groups 1–4 represent the following conditions: 1 si-NC; 2 si-NC + ox-LDL; 3 si-LY86; and 4 si-LY86 + ox-LDL. Data are presented as mean ± SD (n = 3); ^*P<0.05, ^**P<0.01, ^***P<0.001