Fig. 5

The effect of endoplasmic reticulum stress inhibitor 4-PBA on the expression of SREBP2 and HMGCR protein in THP-1 macrophages induced by ox-LDL. (A-B) Immunofluorescence staining of partial aortic arch in mice (GRP78). (C-G) The cropped blot and quantification of SREBP2, HMGCR, GRP78, and LY86 protein in THP-1 macrophages under the effect of 4-PBA on LY86 knockdown and ox-LDL. (H) THP-1 cells stained with oil red O (× 40). (I) Statistical analysis of Oil Red O staining results; a-f represent groupings, a si-NC, b si-NC + ox-LDL, c si-NC + ox-LDL + 4-PBA; d si-LY86, e si-LY86 + ox-LDL, f si-LY86 + ox-LDL + 4-PBA. The findings were presented as mean ± SD (n = 3); ^*P<0.05, ^**P<0.01, ^***P<0.001