Fig. 1

β-catenin is localized in PMs and is expressed at high levels in MCT-induced PH. A-B Double immunofluorescence staining showing the localization of β-catenin expressed on macrophages in MCT-induced lungs. A Paraffin sections of lung tissue and B primary macrophages cultured by alveolar lavage. The top panels show low-magnification images of DAPI (blue), β-catenin (red), and CD68 (green) staining. Scale bar = 50 μm. The bottom panels present high-magnification insets of the yellow boxes in the top panels. C Western blots and quantification analysis showing the protein levels of β-catenin in lung tissue and primary pulmonary macrophages. The blots were cropped and hybridized with the corresponding antibodies. Quantitative Western blot results were normalized to those of β-actin. D Immunofluorescence staining and quantification analysis showing the expression of β-catenin (red). The results of quantitative immunofluorescence staining were normalized to those of DAPI. Sham, Sham group; Sham + XAV939, Sham + XAV939 group; MCT, MCT group; MCT + XAV939, MCT + XAV939 group. The data are presented as the means ± SDs; ns P ≥ 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; n = 3/group