Fig. 3
From: Effect of NLRP3 gene knockdown on pyroptosis and ferroptosis in diabetic cardiomyopathy injury
Establishment and verification of a stable sh-NLRP3 cell line. H9C2 cells were transfected with three target shRNA vectors of NLRP3 (sh-NLRP3#1, #2 and #3) and negative control vector (sh-NC). (A) Features of H9C2 cardiac cells under optical microscopy (100×, scale bar = 100 μm). (B) NLRP3 protein levels in H9C2 cardiac cells normalized by GAPDH levels. (mean ± SD, n = 3). *P < 0.05, **P < 0.01 vs. CON