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Fig. 4 | BMC Cardiovascular Disorders

Fig. 4

From: Nitrogen-containing bisphosphonate induces enhancement of OPG expression and inhibition of RANKL expression via inhibition of farnesyl pyrophosphate synthase to inhibit the osteogenic differentiation and calcification in vascular smooth muscle cells

Fig. 4

Effect of N-BP on VSMCs calcification. VSMCs were preincubated for 2 h with 1 µM or 5 µM ZOL, then VSMCs were co-cultured with or without 10 mM β-GP for 72 h. (A) Alizarin Red S Staining was used to assess VSMCs calcification. Representative images showed VSMCs calcification with purple-red spots. Scale bar = 10 μm. (B) Calcium content was detected by Calcium Assay Kit. Statistical significance was analyzed by one-way ANOVA (*p < 0.05, **p < 0.01, ****p < 0.0001). The data is represented as mean ± SD (n = 3)

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BMC Cardiovascular Disorders

ISSN: 1471-2261

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