Table 1 Polymorphic SNPs markers, PCR primers, restriction enzymes and corresponding alleles
From: Polymorphisms of CD247 gene is associated with dilated cardiomyopathy in Chinese Han population
Marker | Primer sequence (5’- -3’) | Product (bp) | Annealing temperature(0C) | Restriction enzyme | Allele(bp) |
|---|---|---|---|---|---|
rs12141731 | CCACCCGACTGGAGTCAA | 161 | 37 | HincII | T (161) |
GAAGACCCGCCTTCTTTCTT | C (145, 16) | ||||
rs858543 | CAGATGGGCAGGATAGGAAG | 160 | 37 | MboI | C (160) |
TTTCTCCCTGGTTCCTGCTA | T(126, 34) |