Fig. 2

Analysis of mitochondria morphology, lipid droplet accumulation and autophagy in isolated cardiomyocytes using TEM. A) TEM images of cardiomyocytes isolated from mice that were untreated or treated with LPS (5 mg/kg). Red arrows show autophagic events, the orange arrow shows a lipid droplet, and green arrows show mitochondria with disorganized cristae. Images are representative for n = 3 mice/group. B) Mitochondrial area per 50um². C) Percentage of cristae occupancy relative to total mitochondria area. D) Number of mitochondria per 50um².E) Percentage of mitochondria with disorganized cristae relative to the number of mitochondria per 50um². F) Lipid droplet area per 50um²G) Number of autophagic events per 50um². In B-C,values were expressed as mean ± SD and analyzed by a Mann-Whitney U Test. In D-G,values were expressed as mean ± SD and analyzed by a Student’s t-test. Significance was determined by a p-value < 0.05. A value of < 0.05 is indicated by a single asterisk (*), while values of < 0.005, < 0.0005, and < 0.00005 aredenoted by two, three, and four asterisks (**, ***, ****), respectively