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Fig. 3 | BMC Cardiovascular Disorders

Fig. 3

From: An optimized Langendorff-free method for isolation and characterization of primary adult cardiomyocytes

Fig. 3

Assessment of mitochondrial membrane potential in isolated cardiomyocytes. (A) Confocal imaging of JC-1 in in untreated and LPS treated cardiomyocytes (10ng/ml). The scale bar in the upper left panel equals 100 μm and is valid for all panels. Images are representative of 50–70 cardiomyocytes analyzed from n = 3 mice/group. (B) Quantification of JC-1 fluorescence in untreated and LPS treated cardiomyocytes. Values were expressed as mean ± SD and analyzed by a Mann-Whitney U Test. Significance was determined by a p-value < 0.05. **** indicates p < 0.00005

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BMC Cardiovascular Disorders

ISSN: 1471-2261

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