Fig. 4

Analysis of ROS production in isolated cardiomyocytes. (A) Confocal imaging of MitoSOX Red and MitoTracker Green in untreated and LPS treated (10 ng/ml) cardiomyocytes. The scale bar in the upper left panel equals 100 μm and is valid for all panels. Images are representative of 50–70 cardiomyocytes analyzed from n = 3 mice/group. (B) Quantification of MitoSOX Red fluorescence in untreated and LPS treated cardiomyocytes. Images are representative of 50–70 cardiomyocytes analyzed from n = 3 mice/group. Values were expressed as mean ± SD and analyzed by a Mann-Whitney U Test. Significance was determined by a p-value < 0.05. **** indicates p < 0.00005