An optimized Langendorff-free method for isolation and characterization of primary adult cardiomyocytes

Table 4

Compound	Stock Concentration	Final Concentration	µl/1 ml media required
M199 (ThermoFisher Scientific, 11043023)			880
FBS	100%	10%	100
BDM	1 M	10 mM	10
P/S	100×	1×	10

*note: To make 1 M 2,3-Butanedione monoxime (BDM) solution, dissolve 0.101 g of BDM in 1 ml of distilled water and incubate the solution at 37 °C with gentle shaking until BDM is fully dissolved

ISSN: 1471-2261