Fig. 2

Analysis of exosomes in the EAT of canines. (A) Representative electron microscopy image of exosomes isolated from EAT (n = 3, scale bar, 100 nm). (B) Representative NTA image of the exosome size range and concentration (n = 3; dilution ratio, 1:500). (C, D) Representative image of Ad-CD63-RFP immunofluorescence and the mean intensity of Ad-CD63-RFP in EAT (n = 6, x400). (E) Representative western blotting images of CD63, CD81 and TSG101 in EAT. (F-H) The mean expression levels of CD63, CD81 and TSG101 in EAT (n = 6 for each group). *** P < 0.001 versus the sham group; ### P < 0.001 versus the pacing group. EAT = epicardial adipose tissue; NTA = nanoparticle tracking analysis