Fig. 1

SIRT1 inhibition and palmitic acid (PA)-induced insulin-resistant H9c2 cells exhibited elevated lysine (K)-acetylation. (A) The representative original Western Blot image to determine the total K-acetylation level in control (C) cells incubated with and without SIRT1 inhibitor (EX527;10µM), PA-treated insulin-resistant cells incubated with and without SIRT1 activator (SRT1720;2µM) for 24 h. (B) The mean values of total K-acetylation protein levels are given as bar graphs after divided by total protein levels (Supplementary File). EX527 was used as an inhibitor, and SRT1720 as an activator of SIRT1. Bars are represented as mean (± SEM). N = 7–10. Significance level accepted at *p < 0.05 vs. C, and ^†p < 0.05 vs. PA