Fig. 3
From: Cardioprotective role of SIRT1 activation on mitochondrial function in insulin-resistant H9c2 cells
The role of SIRT1 inhibition and palmitic acid (PA)-induced insulin resistance on the levels of reactive oxygen and nitrogen species (ROS and RNS). ROS and RNS are measured in control (C) cells incubated with and without SIRT1 inhibitor (EX527;10µM), PA-treated insulin-resistant cells incubated with and without SIRT1 activator (SRT1720;2µM) for 24 h. (A) The confocal images representative of ROS measurements and (B) their mean values depicted as a bar graph (fold change). (C) The confocal images representative of RNS measurements and (D) their mean values depicted as a bar graph (fold change). EX527 was used as an inhibitor, and SRT1720 as an activator of SIRT1 Bars are represented as mean (± SEM). The total number of cells used per group; ncells=15–25. Significance level accepted at *p < 0.05 vs. C, and †p < 0.05 vs. PA