Fig. 5

The role of SIRT1 inhibition and palmitic acid (PA)-induced insulin resistance on mitochondrial dynamics and apoptosis. (A) Representative Western blot images of Mfn1 (measured at 86 kDa), Mfn2 (measured at 86 kDa), and β-Actin (measured at 43 kDa). (B) Mean values of Mfn1, (C) Mfn2, (D) Mfn1/Mfn2 ratio are given as bar graph. (E) Representative Western blot images of Fis1, and β-Actin measured at 86 kDa and 43 kDa, respectively. (F) Mean value of Fis1 protein level is given as bar graph (G) The relative densitometric changes in the protein ratio of Bax (measured at 21 kDa) to Bcl-2 (measured at 26 kDa) and (H) mean values in the groups of cells are given as bar graph. EX527 was used as an inhibitor, and SRT1720 as an activator of SIRT1. Bars represent as mean (± SEM). N = 5–10. Significance level accepted at *p < 0.05 vs. C, and ^†p < 0.05 vs. PA