Identification of the CeRNA axis of circ_0000006/miR-483-5p/KDM2B in the progression of aortic aneurysm to aorta dissection

Table 1 Animal experimental condition and treatment

Group	Treatment
Control	Fed regular chow diet and drinking water
Model	Fed regular diet + 0.1 g/kg/day BAPN for 28 days, then subcutaneous Angiotensin II injections for 16 days (1.5 mg/kg/day on days 1–7, 0.75 mg/kg/day on days 8–14, 0.375 mg/kg/day on days 15–16)
Model + sh-NC	Model group + 0.2 mL AAV carrying scrambled shRNA (UUCUCCGAACGUGUCACGU) on day 36
Model + sh-circ_0000006	Model group + 0.2 mL AAV carrying circ_0000006 shRNA (AAAUCAAACCAUCAAUCUCUC) on day 36

Notes for Table 1:
AAVs were produced at 1000 pfu/mL and injected around the thoracic aorta arch three times
For Model, Model + sh-NC, and Model + sh-circ_0000006 groups, 15 mice per group were used for aortic aneurysm induction
On day 36, 6 mice per group with confirmed aortic aneurysm growth were included for observing further aortic dissection
Aortic aneurysm was detected by injecting 100 µL ExiTron nano 12,000/25 g mouse and Micro-CT visualization
Mice were sacrificed two weeks after AAV administration for histological analysis of thoracic aortas

ISSN: 1471-2261