Skip to main content
Fig. 3 | BMC Cardiovascular Disorders

Fig. 3

From: Neurogenic differentiation 2 promotes inflammatory activation of macrophages in doxorubicin-induced myocarditis via regulating protein kinase D

Fig. 3

Protein kinase D is involved in the DOX-induced inflammatory activation of macrophages. Macrophages were isolated from the heart tissues of mice in different groups. (A) The purity of macrophages was determined by a FACSAria II flow cytometer. (B-C) The mRNA expressions and protein levels of PKD were detected using qPCR analysis and western blotting. (D-G) qPCR and ELISA assay were carried out to measure the levels of TNF-α, IL-6, IL-18, and IL-1β in macrophages. (H) The protein levels of NLRP3, p-p65, and 65 in each group. (I) The expression of NLRP3 was analyzed in macrophage using qPCR analysis. Values were presented as the mean ± SD of triplicate experiments. Compared with the control group, *P < 0.05, **P < 0. 01, ***P < 0. 001; Compared with DOX + DMSO group, #P < 0.05, ##P < 0. 01, ###P < 0. 001

Back to article page

BMC Cardiovascular Disorders

ISSN: 1471-2261

Contact us