Fig. 4
Expression changes of key apoptosis-related genes in FMC84 cells after 6 h of Tubercidin treatment under varying oxygen and serum conditions. A Western blot analysis showing the relative expression levels of the antiapoptotic gene RFFL in FMC84 cells. After 6 h of Tubercidin treatment (5 μg/ml), the expression of RFFL significantly decreased across all conditions tested (21% O2 + 10% FBS, 21% O2 + 1% FBS, 1% O2 + 10% FBS, 1% O2 + 1% FBS) compared to the untreated controls. GAPDH was used as a loading control. B Western blot analysis showing the relative expression levels of the DNA repair and antiapoptotic gene RIF1 in FMC84 cells. The expression of RIF1 was significantly reduced after 6 h of Tubercidin treatment under all tested conditions compared to the untreated controls. GAPDH was used as a loading control. C Western blot analysis showing the relative expression levels of the proapoptotic gene RNF144B in FMC84 cells. The expression of RNF144B was significantly increased after 6 h of Tubercidin treatment under all tested conditions compared to the untreated controls. GAPDH was used as a loading control. * Statistical significance compared to untreated controls (P < 0.05)