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Fig. 5 | BMC Cardiovascular Disorders

Fig. 5

From: Alkbh5 promotes Ythdf1 expression through demethylation thereby facilitating Fth1 translation to inhibit ferroptosis of myocardial infarction

Fig. 5

Ythdf1 knockdown promoted ferroptosis in the H/R cell model improved by Alkbh5 overexpression. (A) Ythdf1 expression was measured by qPCR. (B) Western blot was performed to measure the protein level of Fth1 in H9C2 cells of different groups. (C) LDH release was measured using a LDH release assay kit. (D) The Fe2+ level was measured using a ferrous ion content assay kit. (E) The GSH level was measured using a GSH content assay kit. (F) The lipid ROS level was measured by C11-BODIPY. (G) The MDA level was measured using a lipid peroxidation MDA assay kit. (H) Apoptosis was assessed by TUNEL assay

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BMC Cardiovascular Disorders

ISSN: 1471-2261

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