Table 1 The primers for qPCR
Gene | Forward Primer | Reverse Primer |
|---|---|---|
Mettl3 | AATGTGCAGCCCAACTGGAT | CTGTGCTTAAACCGGGCAAC |
Mettl14 | AGTGCCGACAGCATTGGTG | GGAGCAGAGGTATCATAGGAAGC |
Wtap | TTTACGCCTGATAGCCAAACA | TCCAAGCTCTTGGTTCTCCTG |
Rbm15 | GGCTCACATACACGGCTTCT | GATGAGCGCGTAGCCTTTGT |
Fto | CACTTGGCTTCCTTACCTGACCCCC | GGAGCAGAGGTATCATAGGAAGC |
Alkbh5 | ACCCGCAAGGTGAAGATGAG | GCCTTAATGGGCAACAAAGCA |
Virma | TCTCTTTAGGTATCTTCACAGCC | AAAGGCATCATCAACCCCGT |
Igf2bp1 | CGTGGAGGCTTTGGGTCT | TGATGTTTCGGATGGTGGC |
Igf2bp2 | CCCCCTATCACCCATTTGCT | TCGAGCCAGCTGTTTGATGT |
Igf2bp3 | GCTGGGCTCGTCGCTAGG | GCCGGTGGATTTTGTCCCT |
Fth1 | TACCTGAATGAGCAGGTGAAAG | GATATTCCGCCAAGCCAGAT |
Ythdf1 | GACAATGACTTTGAGCCCTACCTTTCT | TTGTCCATAGGTAGTGAGATACGGGA |
GPX4 | ATTCCCGAGCCTTTCAACCC | GGTTTTGCCTCATTGCGAGG |
ACSL4 | GAGGCAGCACCTTCGATCC | ACAAGTGGACAGGCAGCAAT |
GAPDH | ACAGCAACAGGGTGGTGGAC | TTTGAGGGTGCAGCGAACTT |