The frequencies of CYP2C19*2, *3, and *17 alleles and their impact on the clinical efficacy of doubled maintenance dose of clopidogrel in Syrian patients with coronary artery disease

Table 1 Primers sequences and PCR conditions

Allele	Primers		PCR Conditions				Product Size
Allele	Primers		Step	Tm^oC	Time	# of Cycles	Product Size
CYP2C192*	F	5′-ACAACCAGAGCTTGGCATATT-3′	Initial Denaturation	95	5 min		203 bp
			Denaturation	95	20 s	40
			Primer Annealing	54.4	30 s
	R	5′-TGTCCATCGATTCTTGGTGT-3′	Extension	72	30 s
	R	5′-TGTCCATCGATTCTTGGTGT-3′	Final Extension	72	10 min
CYP2C193*	F	5′-ATTGTTTCCAATCATTTAGCTTCAC-3′	Initial Denaturation	94	5 min		269 bp
			Denaturation	94	1 min	37
			Primer Annealing	52	45 s
	R	5′-ACTTCAGGGCTTGGTCAATA-3	Extension	72	30 s
	R	5′-ACTTCAGGGCTTGGTCAATA-3	Final Extension	72	5 min
CYP2C1917*	F	5′-GCCCTTAGCACCAAATTCTC-3′	Initial Denaturation	95	3 min		473 bp
			Denaturation	94	1 min	35
			Primer Annealing	56.3	1 min
	R	5′-ATTTAACCCCCTAAAAAAACACG-3′	Extension	72	1 min
	R	5′-ATTTAACCCCCTAAAAAAACACG-3′	Final Extension	72	10 min

ISSN: 1471-2261