Fig. 2

(A) qPCR analysis of PTX3 mRNA expression in cardiomyocytes, normalized to GAPDH (n = 3 biological replicates). (B) qPCR analysis of NLRP3 mRNA expression in cardiomyocytes, normalized to GAPDH (n = 3 biological replicates). (C) Representative Western blot image showing PTX3 and NLRP3 protein expression in cardiomyocytes. (D) Quantitative analysis of PTX3 protein expression relative to GAPDH (n = 3 independent experiments). (E) Quantitative analysis of NLRP3 protein expression relative to GAPDH (n = 3 independent experiments). Data are presented as mean ± SEM